en <span style="font-size:10pt"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Aims: </span></b><span cambria="" style="font-family:">Periodontitis considers one of the essential complications that are accompanied by diabetes mellitus disease. Subsequently, researchers are focusing on the early detection for the development of this disorder by employing suitable biochemical factors as an optimal marker. Accordingly, the current study aimed to assess the levels of both osteopontin and alkaline phosphatase in patients Sera who suffering from diabetes with periodontitis to knowledge their ability to be the best factors for early prediction. </span><b><span style="font-family:"Cambria","serif""></span></b></span></span></span></span><br> <span style="font-size:10pt"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Material & Methods</span></b><b><span cambria="" style="font-family:">:</span></b><span cambria="" style="font-family:"> Blood samples were collected from 120 male persons, the age range of 35-65 years, in the Al-Baladiyat specialist center for dentistry. Patients were divided into three groups (30 with diabetic mellitus type 2, 30 with periodontitis, 30 patients with diabetic Mellitus with periodontitis) and 30 apparently healthy subjects as a management group. Serum levels of Fasting Blood Sugar and Alkaline phosphatase were estimated enzymatic method. Osteopontin levels were measured by the ELISA method. </span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Findings:</span></b><span cambria="" style="font-family:"> The results showed an increase in </span><a href="https://en.wikipedia.org/wiki/Osteopontin"><span cambria="" style="font-family:"><span style="text-decoration:none"><span style="text-underline:none">osteopontin</span></span></span></a><span cambria="" style="font-family:"> levels in patients groups of diabetes mellitus and diabetes mellitus with periodontitis compared to patients with only periodontitis and control groups. Also, results revealed a highly significant increase in Alkaline phosphatase levels in two patient groups including diabetic and diabetic with periodontitis in comparison with periodontitis and healthy groups. Receiver operating characteristic data analysis showed high values of the studied parameters in terms of AUC.</span></span></span></span><br> <b><span style="font-size:10.0pt"><span cambria="" style="font-family:">Conclusion:</span></span></b><span style="font-size:10.0pt"><span cambria="" style="font-family:"> Analysis data by Receiver operating characteristic curve confirmed that both </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><a href="https://en.wikipedia.org/wiki/Osteopontin"><span cambria="" style="font-family:"><span style="text-decoration:none"><span style="text-underline:none">Osteopontin</span></span></span></a></span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> and Alkaline phosphatase consider excellent markers for the diagnosis of the studied cases based on the values of AUC. Accordingly, </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><a href="https://en.wikipedia.org/wiki/Osteopontin"><span cambria="" style="font-family:"><span style="text-decoration:none"><span style="text-underline:none">osteopontin</span></span></span></a></span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> was found to be the best marker that can be employed in the diagnosis of the studied cases</span></span><br> <span style="font-size:11pt"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:8.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span></span> Diabetes Mellitus,Periodontitis,Osteopontin,Alkaline Phosphatase, 105 109 http://ijwph.daneshafarand.org/browse.php?a_code=A-10-1909-1&slc_lang=en&sid=3 Z.A. Salman 1003194753284600378420 1003194753284600378420 Yes Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq K.K. Ghudhaib 1003194753284600378419 1003194753284600378419 No Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq