en <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Aims:</span></b> <span cambria="" style="font-family:">This study aimed to detect gram-positive bacteria that cause UTIs with multiple antibiotic resistance, as well as phenotype and genotype methods to determine some genes in<i> S. aureus</i>&nbsp;isolates. </span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:"><span style="color:black">Materials & Methods:</span></span></b><span cambria="" style="font-family:"><span style="color:black"> This study included 205 urine samples from outpatients with a urinary tract infection. The Polymerase Chain Reaction method was performed to evaluate the genotypic status of the <i>16S rRNA, erm B, mecA, bla Z</i>, and <i>msrA</i> genes after DNA extraction. </span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:"><span style="color:black">Findings:</span></span></b><span cambria="" style="font-family:"><span style="color:black"> only </span></span><span cambria="" style="font-family:">51 (41.4%)<span style="color:black"> were found to have gram-positive bacteria, isolated bacteria were </span>divided into two groups that include 37 isolates (30.08%) were</span><span cambria="" style="font-family:"> <i>Staphylococcus</i> spp. </span><span cambria="" style="font-family:">and 14 isolates<i> </i>(11.3%) diagnosed as <i>Enterococcus</i></span><span cambria="" style="font-family:"> spp. and <i>Staphylococcus</i> spp. </span><span cambria="" style="font-family:">divided into two groups that include 17 isolates (13.8%) diagnosed as</span><span cambria="" style="font-family:"> <i>S. aureus</i> and 20 isolates (16.2%)</span><span cambria="" style="font-family:"> diagnosed as <span style="background:white"><span style="color:#202124">coagulase-negative staphylococci</span></span></span><span cambria="" style="font-family:">, distributed as 7 isolates (5.6%) </span><span cambria="" style="font-family:">diagnosed as</span><span cambria="" style="font-family:"> <i>S. haemolyticus</i>, 3 isolates (3.4%) </span><span cambria="" style="font-family:">diagnosed as</span><span cambria="" style="font-family:"> <i>S. epidermidis</i>, 1 isolate (0.8%) </span><span cambria="" style="font-family:">diagnosed as</span><span cambria="" style="font-family:"> <i>S. vitulinus</i>,</span><span cambria="" style="font-family:">4 isolates (3.2%) diagnosed as</span><span cambria="" style="font-family:"> <i>S. sciuri</i>, </span><span cambria="" style="font-family:">3 isolates (2.4%) diagnosed <i>as</i></span><i><span cambria="" style="font-family:"> S. homins</span></i><i><span cambria="" style="font-family:">, </span></i><span cambria="" style="font-family:">1 isolate (0.8%) diagnosed as</span><span cambria="" style="font-family:"> <i>S. warneri</i>,</span><i> </i><span cambria="" style="font-family:">1 isolate (0.8%) diagnosed as</span><span cambria="" style="font-family:"> <i>S. lentus. </i></span><span cambria="" style="font-family:"><span style="color:black"></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:"><span style="color:black">Conclusion:</span></span></b><span cambria="" style="font-family:"><span style="color:black"> The most common gram-positive bacteria found to cause UTIs was </span></span><i><span cambria="" style="font-family:">S. aureus</span></i><i><span cambria="" style="font-family:"><span style="color:black">.</span></span></i><span cambria="" style="font-family:"><span style="color:black"> A few pathogens of<i> S. aureus</i> were discovered to be resistant to Vancomycin and linezolid in this study. The prevalence of <i>blaZ</i> genes, which are responsible for resistance, was found to be high among pathogenic <i>S. aureus </i>isolates in Diyala province.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="unicode-bidi:embed"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span dir="RTL" lang="AR-IQ" style="font-size:14.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""></span></span></span></b></span></span></span></span> Uropathogenic Escherichia coli,Staphylococcus aureus,Urinary Tract Infections,Bacterial Proteins, 99 104 http://ijwph.daneshafarand.org/browse.php?a_code=A-10-1910-1&slc_lang=en&sid=3 Sh. Ahmed Khalaf 1003194753284600378543 1003194753284600378543 Yes Pharmacy Department, Bilad Alrafidain University College, Faculty of Pharmacy & Medical Sciences, Al Ahliyya Amman University, Iraq H.K. AL-Tameemi 1003194753284600378422 1003194753284600378422 No Medical Laboratory Technologies Department, Bilad Alrafidain University College, Al Ahliyya Amman University, Iraq Y. Jasem Abdullah 1003194753284600378421 1003194753284600378421 No Medical Labs Department, Amara Technical Institute, Southern Technical University, Amara, Iraq