en <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Aims: </span></b><span cambria="" style="font-family:">Bone defect repair is still a serious clinical orthopedic issue. To preserve skeletal integrity, bone is a highly vascularized tissue that relies on a close spatial and temporal interaction between blood vessels and bone cells. The aim of the study was to see if the local application of vitronectin protein /angiopoietin-like 4 protein as an autoinducer could help with bone repair.</span><b><span style="font-family:"Cambria","serif""></span></b></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Material & Methods: </span></b><span cambria="" style="font-family:">In this study, 48 albino male rats weighing 300-400 grams and aged 6-8 months were employed under temperature, drinking, and food consumption control conditions. The animals were subjected to a surgical procedure on the medial side of the tibiae bone. In the control group, the bone defect was treated with a local application of an absorbable hemostatic sponge, whereas in the experimental group, which was divided into three subgroups, a group I the bone defect was treated with a local application of 1mg vitronectin protein, group II with a local application of 1mg angiopoietin-like 4 protein, and group III with a local application of a combination of the two (0.5mg vitronectin protein and 0.5 mg of angiopoietin-like 4). The absorbable hemostatic sponge was used to fix all of the experimental groups. 14 and 28 days following surgery, the rats were slaughtered (six rats for each period).</span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Findings:</span></b> <span cambria="" lang="EN-GB" style="font-family:">In comparison to other groups, bone defects treated with local application of both vitronectin and angiopoietin protein exhibit new bone formation with a high bone cell count and high bone architectures in terms of trabecular number, bone marrow area, and bone marrow space</span><span cambria="" style="font-family:">. </span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Conclusion:</span></b> <span cambria="" lang="EN-GB" style="font-family:">The findings of this investigation revealed that a combination of vitronectin and angiopoietin-like proteins exhibited potential efficacy in boosting bone defect healing</span><span cambria="" style="font-family:">. </span></span></span></span><br> &nbsp; vitonectin protein,angiopoietin likeprotein4 231 242 http://ijwph.daneshafarand.org/browse.php?a_code=A-10-1919-1&slc_lang=en&sid=3 E. Fadhil Kadhim 1003194753284600378454 0000000336980349 Yes College of Dentistry University Baghdad, Baghdad, Iraq