en <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Aims:</span></span></span></b><span style="font-size:10.0pt"><span cambria="" style="font-family:"> One extremely important public health challenge worldwide is diabetes mellitus.</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> Type 2 diabetes mellitus is a rising concern around the globe. Early revealing of Type 2 diabetes mellitus is very significant in that morbidity associated with the condition can be decreased.</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> Studies have proposed that insulin-like growth factor-1 has roles similar to that of insulin. The existing study was implemented to reveal the alliance of single nucleotide polymorphism rs10860860 of the <i>IGF</i>-<i>1</i> gene with </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">Type 2 diabetes mellitus</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Materials & Methods:</span></span></span></b><span style="font-size:10.0pt"><span cambria="" style="font-family:"> This case-control study was conducted in Merjan Hospital in Babylon Province, Iraq, in 2022. The available random sampling method selected two groups of healthy individuals (n=50 as control) and </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">Type 2 diabetes mellitus</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> patients (n=158 as study). </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">The serum levels of </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">glucose and zinc </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">were determined</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> by spectrophotometric method. </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">The serum IGF-1 level was estimated using the sandwich ELISA technique.</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> The </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">students&rsquo; T and Chi-square tests </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">were used to assess data </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">in SPSS 21 software</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">.</span></span><span style="font-size:10.0pt"><span style="font-family:"Cambria",serif"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Findings:</span></span></span></b><span style="font-size:10.0pt"><span cambria="" style="font-family:"> The different levels of glucose, zinc, and IGF-1 were significant between the two groups</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">. Genotyping frequencies of the </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">IGF-1</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> gene deviated from </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">Hardy Weinberg equilibrium </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">in the control group</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> (&chi;²=</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">9.158</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">; p=</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">0.002)</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> while were consistent in the study group (</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">&chi;²=</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">2.482</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">; p=</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">0.115</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">)</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">. </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">The </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">AT</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> genotype was associated with lower odds of </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">Type 2 diabetes mellitus</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:"> than those of the wild genotype AA. </span></span>&nbsp;&nbsp;</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span cambria="" style="font-family:">The AT genotype of IGF-1 gene SNP rs10860860 negatively associates with </span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">Type 2 Diabetes Mellitus</span></span><span style="font-size:10.0pt"><span cambria="" style="font-family:">.</span></span></span></span></span></span> Polymorphism,Insulin-like Growth Factor-1,Diabetes Mellitus, Type 2, 305 310 http://ijwph.ir/browse.php?a_code=A-10-2117-1&slc_lang=en&sid=3 M.A. AbdulAemah 1003194753284600379125 1003194753284600379125 Yes Department of Clinical Laboratory Sciences, College of Pharmacy, Al-Mustaqbal University, Hillah, Iraq G.M. Hussein 1003194753284600378896 1003194753284600378896 No Department of Clinical Laboratory Sciences, College of Pharmacy, AL-Zahraa University for Women, Karbala, Iraq N.Y. Hussein 1003194753284600378897 1003194753284600378897 No Department of Pharmacy, Al-Manara College for Medical Sciences, Amarah, Iraq