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en Cytotoxin Production and Slim Layer Formation by Methicillin-Resistant Staphylococcus auras Isolated from Diabetic Patients Aims: Methicillin-resistant Staphylococcus aureus frequently causes infection of diabetic foot ulcers. However, no investigations have investigated the connection between methicillin-resistant A, perivascular tissue neoplasia, and delayed healing of diabetic foot ulcers. The purpose of this study was to genetically isolate methicillin-resistant from diabetic foot ulcer patients and define its function in the development of chronic ulcer lesions, the course of the disease, and antibiotic resistance. Materials & Methods: This experimental study was conducted on patients referred to the Diabetes Endocrinology Center in Al-Basrah, Southern Iraq, from 2019 to 2020. Only 31 isolates were found in the 80 samples of diabetic foot ulcer patients; 12 Staphylococcus aureus (38.7%) and 19 other Staphylococcus spp. (61.3%). All 12 samples of Staphylococcus aureus strains were confirmed by amplifying the universal 16SrRNA gene for pak2, MR30, CFSAN007896, and ST4. Findings: The mecA gene was 100% positive for the icaA gene (involved in biofilm formation) and 75% positive for the pol gene (involved in the manufacture of cytotoxins), revealing that all 12 Staphylococcus aureus isolates were methicillin-resistant. Conclusion: Panton-valentine leukocidin has a high prevalence among MRSA strains isolated from diabetic foot ulcer patients that form biofilms, causing patients to have significant inflammation, illness progression, and challenging wound healing, which may result in lower limb amputations.   Diabetic Foot,Methicillin Resistance Staphylococcus aureus,Cytotoxin,Biofilm,Universal 16srdna Gene, 415 420 http://ijwph.daneshafarand.org/browse.php?a_code=A-10-2038-2&slc_lang=en&sid=3 K.A. Hussein 1003194753284600379225 1003194753284600379225 No Department of Medical Sciences, College of Nursing, University of Basrah, Basrah, Iraq K.S. Madhi 1003194753284600379223 1003194753284600379223 No Department of Human Anatomy, College of Medicine, University of Basrah, Basrah, Iraq Z.A. Al-Mayyahi 1003194753284600379224 1003194753284600379224 No Department of Medicine, College of Medicine, University of Basrah, Basrah, Iraq N.A. Al-Ammar 1003194753284600379222 1003194753284600379222 No Department of Microbiology, College of Medicine, University of Basrah, Basrah, Iraq B.A. Abbas 1003194753284600379152 1003194753284600379152 Yes Department of Microbiology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq A.H. Faraj 1003194753284600379901 1003194753284600379901 No Public Health Laboratory, Sulaymaniyah, Iraq