en <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Aims: </span></span></span></b><span style="font-size:10.0pt"><span cambria="" style="font-family:">Garden cress (<i>Lepidium sativum</i> L.), a member of the Cruciferae family, is widely planted worldwide, especially in India, Europe, and the United States. One of the most common mosquito species that carries <i>Plasmodium falciparum</i> is the <i>Anopheles gambiae</i> sensu lato, which may be combated by sprinkling fields with <i>Lepidium sativum</i> seeds. This study aimed to test the <i>Lepidium sativum</i> Linn. seeds extract as an antimicrobial agent against food pathogens. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Materials & Methods:</span></span></span></b> <span style="font-size:10.0pt"><span cambria="" style="font-family:">The active components were extracted from the powdered dry seeds using chloroform, ethyl acetate, methanol, and dichloromethane. The antibacterial activity of various doses of the extracts was evaluated using agar well diffusion. The MIC and MBC for the most effective extract were estimated using tube dilution and subculturing methods. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Findings:</span></span></span></b><span style="font-size:10.0pt"><span cambria="" style="font-family:"> Staphylococcus aureus had an MIC of 1.56mg/ml and a minimum inhibitory concentration (MBC) of 6.52mg/ml, while Salmonella typhi had an MIC of 25mg/ml. At a concentration of 10% <i>L. sativum</i>, the half-life (KDT₅₀) was determined to be 7.34 minutes. Conversely, the half-life of <i>L. sativum</i> oil was only 10.05 minutes when used at the same concentration. Compared to the <i>L. sativum</i> concentrations that resulted in the KDT₅₀ (50 and 10%, respectively), the bendiocarb 0.1% impregnated filter paper performed poorly. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Conclusion: </span></span></span></b><i><span style="font-size:10.0pt"><span cambria="" style="font-family:">L. sativum</span></span></i><span style="font-size:10.0pt"><span cambria="" style="font-family:"> essential oil effectively kills <i>Anopheles gambiae</i>. </span></span></span></span></span></span><br> &nbsp; Lepidium sativum L.,Antimicrobial,Anti-Mosquito,Anopheles gambiae, 387 394 http://ijwph.daneshafarand.org/browse.php?a_code=A-10-2136-1&slc_lang=en&sid=3 H.A. Emaduldeen 1003194753284600379211 1003194753284600379211 No Environment Research Center, University of Technology, Baghdad, Iraq M.Q. Waheeb 1003194753284600379124 1003194753284600379124 Yes Department of Biology, College of Science, Al Muthanna University, AL Muthanna, Iraq M.A. Makki 1003194753284600379119 1003194753284600379119 No Department of Biology, College of Science, Al Muthanna University, AL Muthanna, Iraq