en <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Aims:</span></span></span></b><span style="font-size:10.0pt"><span cambria="" style="font-family:"> <i>Escherichia coli</i> is a Gram-negative bacteria classified into non-pathogenic and pathogenic serotypes. This study aimed to blend phenotypic and genotypic techniques to identify <i>Escherichia coli</i> isolated from patients and dogs.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Materials & Methods:</span></span></span></b><span style="font-size:10.0pt"><span cambria="" style="font-family:"> 80 samples were collected from humans and dogs with UTI or diarrhea cultured on MacConkey, EMB, and nutrient agars. The suspected <i>E. coli</i> bacteria was confirmed using a PCR-based molecular method. Two bacterial identification techniques were applied to detect the zoonotic transmission possibility; a phenotypic analyzer using Antibiotic Susceptibility test Patterns and molecular genotypic technique depending on the presence of four exact genes in each isolate. </span></span><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:black"></span></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Findings:</span></span></span></b> <span style="font-size:10.0pt"><span cambria="" style="font-family:">All the isolated <i>E. coli</i> were multi-drug and strongly resistant (100%) to Amoxicillin+clavulanic acid, Oxacillin, and Vancomycin. Meanwhile, they were highly susceptible to imipenem (100%). The ASP5 (i) was the dominant pattern among human and animal isolates and the most common shared pattern among the four groups of samples. The results showed that 31 of 50 (62%) isolates have similar ASPs; however, only 16 (53.3%) shared the same phylogenetic groups. Furthermore, the molecular genetic group C is highly prevalent in dog isolates, whereas group E was the commonest among human isolates. </span></span><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:black"></span></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span cambria="" style="font-family:"><span style="color:#f79646">Conclusion:</span></span></span></b><span style="font-size:10.0pt"><span cambria="" style="font-family:"> Both methods would be more accurate and better explain bacterial transmission and acquiring new antibiotic-resistance genes.</span></span></span></span></span><br> &nbsp; Public Health,Zoonotic Diseases,Clermont Phylotyping,Escherichia coli,Antibiotic Sensitivity Profile, 279 287 http://ijwph.daneshafarand.org/browse.php?a_code=A-10-2233-1&slc_lang=en&sid=3 N.F. Almousawi 1003194753284600379508 1003194753284600379508 No Department of Microbiology, College of Medicine, University of Basrah, Basrah, Iraq M.Y. Al-Hejjaj 1003194753284600379509 1003194753284600379509 Yes Department of Microbiology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq