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Volume 15, Issue 1 (2023)
3 2023, 15(1): 101-105 |
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History
Received: 2022/11/26 | Accepted: 2023/03/8 | Published: 2023/03/16
Received: 2022/11/26 | Accepted: 2023/03/8 | Published: 2023/03/16
How to cite this article
Banoon S, Hussein Ali Z, Al-Kraety I, Aziz Z. Molecular Detection of blaTEM and blaCTX-M Encoding Genes from Klebsiella oxytoca Isolates from Tonsillitis. 3 2023; 15 (1) :101-105
URL: http://ijwph.daneshafarand.org/article-3-85505-en.html
URL: http://ijwph.daneshafarand.org/article-3-85505-en.html
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1- Department of Biology, College of Science, University of Misan, Misan, Iraq
2- Alameen Center for Advanced Research and Biotechnology, Imam Ali Holy Shrine, Najaf, Iraq
3- Department of Medical Laboratory Techniques, Faculty of Health and Medical Techniques, University of Alkafeel, Najaf, Iraq
2- Alameen Center for Advanced Research and Biotechnology, Imam Ali Holy Shrine, Najaf, Iraq
3- Department of Medical Laboratory Techniques, Faculty of Health and Medical Techniques, University of Alkafeel, Najaf, Iraq
Abstract (2235 Views)
Aims: Tonsillitis is inflammation of the tonsils, a common clinical state caused by bacterial or viral infections. There are different types of tonsillitis; acute, sub-acute, chronic, and recurrent. The aim of this study was the isolation and identification of Klebsiella oxytoca isolated from tonsillitis based on conventional standard bacteriological methods and confirmed by VITEK-2 compact system.
Materials & Methods: A polymerase chain reaction was performed to detect blaCTX-M and blaTEM genes.
Findings: A total of 50 specimens were recovered from tonsillitis using swab sampling, which contained 35 bacterial growths. Onto the MacConkey agar, 15 isolates were confirmed as K. oxytoca using IMVIC test and VITEK-2 compact system. In the genotypic test, K. oxytoca isolates contained 11 (73.3%) blaCTX-M and 10 (66.6%) blaTEM genes.
Conclusion: The use of the VITEK-2 system is necessary to confirm the precise identification of K. oxytoca nosocomial pathogens from tonsillitis. The existence of blaTEM and blaCTX-M gene in half of K. oxytoca isolates is a concern that needs control strategies.
Materials & Methods: A polymerase chain reaction was performed to detect blaCTX-M and blaTEM genes.
Findings: A total of 50 specimens were recovered from tonsillitis using swab sampling, which contained 35 bacterial growths. Onto the MacConkey agar, 15 isolates were confirmed as K. oxytoca using IMVIC test and VITEK-2 compact system. In the genotypic test, K. oxytoca isolates contained 11 (73.3%) blaCTX-M and 10 (66.6%) blaTEM genes.
Conclusion: The use of the VITEK-2 system is necessary to confirm the precise identification of K. oxytoca nosocomial pathogens from tonsillitis. The existence of blaTEM and blaCTX-M gene in half of K. oxytoca isolates is a concern that needs control strategies.
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