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Volume 18, Issue 3 (2026)
3 2026, 18(3): 1001-1009 |
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Received: 2026/09/10 | Accepted: 2026/09/19 | Published: 2026/09/19
Received: 2026/09/10 | Accepted: 2026/09/19 | Published: 2026/09/19
How to cite this article
Qasim M.J.. In Vitro Antimicrobial Activity of Date Seed Extract Against a Multidrug-Resistant Clinical Isolate of Helicobacter pylori. 3 2026; 18 (3) :1001-1009
URL: http://ijwph.daneshafarand.org/article-3-87225-en.html
URL: http://ijwph.daneshafarand.org/article-3-87225-en.html
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Abstract (14 Views)
Background: Helicobacter pylori remains one of the most prevalent human bacterial pathogens, colonizing approximately 30–70% of the global population and representing a major cause of chronic gastritis, peptic ulcer disease, and gastric adenocarcinoma. This study evaluated the in vitro antibacterial activity of date seed extract against a multidrug-resistant clinical isolate of H. pylor.
Methods: A multidrug-resistant clinical isolate of H. pylori was recovered from a gastric biopsy specimen obtained from a dyspeptic patient and identified by conventional microbiological tests and PCR amplification of the cagA virulence gene. Ethanolic date seed extract was prepared under standardized laboratory conditions. The phytochemical profile of the extract was characterized by GC–MS analysis. Antibacterial activity was evaluated using the agar well diffusion assay, while the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using the resazurin microdilution assay and subsequent subculture, respectively. All experiments were performed in triplicate and expressed as mean ± standard deviation.
Results: Phytochemical profiling identified eighteen major bioactive compounds in the ethanolic extract of Phoenix dactylifera seeds, mainly proanthocyanidins, flavonoid glycosides, catechins, and hydroxycinnamic acid derivatives. Proanthocyanidin tetramer was the predominant constituent. The extract exhibited pronounced antibacterial activity against the multidrug-resistant H. pylori isolate, producing an inhibition zone of 32.0 ± 0.2 mm. The MIC and MBC values were 15.6 ± 0.5 μg/mL and 31.2 ± 0.3 μg/mL, respectively. The observed antimicrobial activity is likely attributable to the synergistic effects of the identified polyphenolic constituents, which may interfere with bacterial membrane integrity and essential cellular processes.
Methods: A multidrug-resistant clinical isolate of H. pylori was recovered from a gastric biopsy specimen obtained from a dyspeptic patient and identified by conventional microbiological tests and PCR amplification of the cagA virulence gene. Ethanolic date seed extract was prepared under standardized laboratory conditions. The phytochemical profile of the extract was characterized by GC–MS analysis. Antibacterial activity was evaluated using the agar well diffusion assay, while the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using the resazurin microdilution assay and subsequent subculture, respectively. All experiments were performed in triplicate and expressed as mean ± standard deviation.
Results: Phytochemical profiling identified eighteen major bioactive compounds in the ethanolic extract of Phoenix dactylifera seeds, mainly proanthocyanidins, flavonoid glycosides, catechins, and hydroxycinnamic acid derivatives. Proanthocyanidin tetramer was the predominant constituent. The extract exhibited pronounced antibacterial activity against the multidrug-resistant H. pylori isolate, producing an inhibition zone of 32.0 ± 0.2 mm. The MIC and MBC values were 15.6 ± 0.5 μg/mL and 31.2 ± 0.3 μg/mL, respectively. The observed antimicrobial activity is likely attributable to the synergistic effects of the identified polyphenolic constituents, which may interfere with bacterial membrane integrity and essential cellular processes.