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Volume 14, Issue 1 (2022)
3 2022, 14(1): 99-104 |
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History
Received: 2022/03/1 | Accepted: 2022/04/12 | Published: 2022/04/9
Received: 2022/03/1 | Accepted: 2022/04/12 | Published: 2022/04/9
How to cite this article
Ahmed Khalaf S, AL-Tameemi H, Jasem Abdullah Y. Detection of Genes ermB, mecA, bla Z and msrA in Uropathogenic Staphylococcus aureus Isolates between the Gram-Positive Bacteria that Cause Urinary Tract Infections. 3 2022; 14 (1) :99-104
URL: http://ijwph.daneshafarand.org/article-3-85454-en.html
URL: http://ijwph.daneshafarand.org/article-3-85454-en.html
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1- Pharmacy Department, Bilad Alrafidain University College, Faculty of Pharmacy & Medical Sciences, Al Ahliyya Amman University, Iraq
2- Medical Laboratory Technologies Department, Bilad Alrafidain University College, Al Ahliyya Amman University, Iraq
3- Medical Labs Department, Amara Technical Institute, Southern Technical University, Amara, Iraq
2- Medical Laboratory Technologies Department, Bilad Alrafidain University College, Al Ahliyya Amman University, Iraq
3- Medical Labs Department, Amara Technical Institute, Southern Technical University, Amara, Iraq
Abstract (3095 Views)
Aims: This study aimed to detect gram-positive bacteria that cause UTIs with multiple antibiotic resistance, as well as phenotype and genotype methods to determine some genes in S. aureus isolates.
Materials & Methods: This study included 205 urine samples from outpatients with a urinary tract infection. The Polymerase Chain Reaction method was performed to evaluate the genotypic status of the 16S rRNA, erm B, mecA, bla Z, and msrA genes after DNA extraction.
Findings: only 51 (41.4%) were found to have gram-positive bacteria, isolated bacteria were divided into two groups that include 37 isolates (30.08%) were Staphylococcus spp. and 14 isolates (11.3%) diagnosed as Enterococcus spp. and Staphylococcus spp. divided into two groups that include 17 isolates (13.8%) diagnosed as S. aureus and 20 isolates (16.2%) diagnosed as coagulase-negative staphylococci, distributed as 7 isolates (5.6%) diagnosed as S. haemolyticus, 3 isolates (3.4%) diagnosed as S. epidermidis, 1 isolate (0.8%) diagnosed as S. vitulinus,4 isolates (3.2%) diagnosed as S. sciuri, 3 isolates (2.4%) diagnosed as S. homins, 1 isolate (0.8%) diagnosed as S. warneri, 1 isolate (0.8%) diagnosed as S. lentus.
Conclusion: The most common gram-positive bacteria found to cause UTIs was S. aureus. A few pathogens of S. aureus were discovered to be resistant to Vancomycin and linezolid in this study. The prevalence of blaZ genes, which are responsible for resistance, was found to be high among pathogenic S. aureus isolates in Diyala province.
Materials & Methods: This study included 205 urine samples from outpatients with a urinary tract infection. The Polymerase Chain Reaction method was performed to evaluate the genotypic status of the 16S rRNA, erm B, mecA, bla Z, and msrA genes after DNA extraction.
Findings: only 51 (41.4%) were found to have gram-positive bacteria, isolated bacteria were divided into two groups that include 37 isolates (30.08%) were Staphylococcus spp. and 14 isolates (11.3%) diagnosed as Enterococcus spp. and Staphylococcus spp. divided into two groups that include 17 isolates (13.8%) diagnosed as S. aureus and 20 isolates (16.2%) diagnosed as coagulase-negative staphylococci, distributed as 7 isolates (5.6%) diagnosed as S. haemolyticus, 3 isolates (3.4%) diagnosed as S. epidermidis, 1 isolate (0.8%) diagnosed as S. vitulinus,4 isolates (3.2%) diagnosed as S. sciuri, 3 isolates (2.4%) diagnosed as S. homins, 1 isolate (0.8%) diagnosed as S. warneri, 1 isolate (0.8%) diagnosed as S. lentus.
Conclusion: The most common gram-positive bacteria found to cause UTIs was S. aureus. A few pathogens of S. aureus were discovered to be resistant to Vancomycin and linezolid in this study. The prevalence of blaZ genes, which are responsible for resistance, was found to be high among pathogenic S. aureus isolates in Diyala province.